Positive reactions were seen in the 1/4, 1/8, and 1/32 dilutions and negative in the 1/64 dilution. A test for the presence of an antibody was then made by adding a hepatitis A antigen to each of the tubes. Final concentration: A hepatitis A antibody titer analysis was executed on a blood sample. A solution that contains 800 mg/dL serum is diluted 1/2, then 1/4, then 1/8, and then again 1/16 in a series. Give the final concentration for the sample. Find the concentration in each test tube. how to calculate the concentration of the diluted solutions if they unknown First: find the D.F: Dilution factor (D.F) final volume / aliquot volume. A dilution series is then carried out with the following dilutions for each tube: 1/2, 1/2, 1/2, 1/2 and 1/2. The results I'm getting are too low though, and i'm afraid that i'm not taking into account the dilution correctly.Calculate the dilution factor for the following problem: 10 mL sample added to 190 mL diluents What is the final dilution of an unknown blood specimen using 0.1 mL blood with 3.4 mL of water and 1.5 mL of reagents? If 20 mL of saline is added to 4 mL of serum, what is the serum dilution? A urine sample has a concentration of 500 mg/dL. I want to measure the amount of HCP in in the 3 peaks, so what i did and I pooled the fractions together (for each peak) and used an assay to measure HCP. I'm collecting 2ml fractions, so I get 15 fractions for the FT, 7 for the Eluate and 1 for the Strip. I get a wide FT flat peak, an elution peak and a tiny peak at the end (strip). I inject 30ml into the 1ml column (at 1ml/min). I take my harvest and dilute it 1:4 in buffer A. i think it's a pretty simple problem but i'm not sure my logic is correct.īackground info: CHO harvest feed is injected onto a 1ml AIEX column using an Akta avant. I have a question about calculating concentration of HCP in fractions collected from an AIEX run and I can't get my head around it.
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